Journal: iScience

Article Title: Construction of in vitro models derived from distinct regions of mouse nasal mucosa

doi: 10.1016/j.isci.2026.117611

Figure Lengend Snippet:

Article Snippet: PD98059 , MedChemExpress , Cat# HY-12028.

Techniques: Recombinant, Tissue, Purification, Software

Journal: Acta Pharmaceutica Sinica. B

Article Title: Hepatokine FGL1 drives endothelial injury and atherogenesis via integrin β 1-mediated endothelial–mesenchymal transition

doi: 10.1016/j.apsb.2026.06.005

Figure Lengend Snippet: FGL1 promotes EndoMT by activating FAK/ERK and SMAD signaling cascades sequentially downstream of integrin β 1 activation. (A) Western blot analysis of the time-dependent activation effects of FGL1 on FAK, ERK and Smad signaling molecules in endothelial cells; n = 3–4. (B) Western blot analysis of the effects of small molecule inhibitors of FAK (GSK2256098) and ERK (PD98059) on FGL1-induced FAK and ERK activation and EndoMT; n = 3–6. (C) Western blot analysis of the effects of small molecule Smad2/3 inhibitor (SB431542) on FGL1-induced Smad2/3 activation and EndoMT; n = 3–4. (D) Western blot analysis of the effects of small molecule inhibitors of FAK (GSK2256098) and Smad2/3 (SB431542) on FGL1-induced upregulation of pro-EndoMT transcriptional factors; n = 3. (E) The administration strategy of recombinant FGL1 protein (15 μg/kg) in Apoe −/− mice. (F) Quantification of plasma FGL1 protein concentration through ELISA analysis; n = 8. (G–I) Immunofluorescence analysis of phospho-FAK(Tyr397), phospho-ERK1/2(Tyr202/204) and phospho-Smad2/3(Ser465/467) in aortic root of atherosclerotic mice; Scale bar: 50 μm; n = 6. Data are presented as mean ± SEM. Each dot represents an average of three technical replicates of a dish of cells or a mouse.

Article Snippet: In the other series of experiments, HUVECs were pretreated with FAK inhibitor GSK2256098 (#T2281, Topscience), MEK inhibitor PD98059 (#T2623, Topscience), ALK inhibitor SB431542 (#T1726, Topscience) for 30 min, or pretreated with Integrin β 1 antibody (#MAB17781, R&D Systems) for 6 h, followed by treatment with FGL1.

Techniques: Activation Assay, Western Blot, Recombinant, Clinical Proteomics, Protein Concentration, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Physiological Reports

Article Title: TLR4 signaling cascades leading to increases in albumin permeability, nephrin ectodomain shedding, and apoptosis in MPC ‐5 podocytes

doi: 10.14814/phy2.71147

Figure Lengend Snippet: Role of intracellular signaling cascades in TLR4‐mediated increase in P Alb . CRX527 exposure for 1 h causes increase in P Alb that was reduced by concurrent exposure to the ROS quencher TEMPOL (a) or by the MEK1 inhibitor PD98059 (b), which blocks activation of Erk1/2. (c) CRX527 exposure for 24 h caused an increase in P Alb that persisted in the presence of JSH‐23, an agent that blocks activation of NFκB signaling. In these graphs, points indicate individual measurements, horizontal bars indicate the mean of the group, and error bars indicate SD.

Article Snippet: JSH 23 (S7351) and PD98059 (S1177) were purchased from Selleckchem (Houston, TX, USA).

Techniques: Activation Assay

Journal: Physiological Reports

Article Title: TLR4 signaling cascades leading to increases in albumin permeability, nephrin ectodomain shedding, and apoptosis in MPC ‐5 podocytes

doi: 10.14814/phy2.71147

Figure Lengend Snippet: Role of intracellular signaling cascades in TLR4‐mediated nephrin ectodomain shedding. CRX527 exposure for 24 h caused an increase in nephrin shedding that was blocked by the Erk pathway inhibitor PD98059 (a). By contrast, this response persisted in the presence of the ROS quencher TEMPOL (b), and in the presence of JSH‐23, an agent that blocks activation of NFκB signaling (c). In these graphs, points indicate individual measurements, horizontal bars indicate the mean of the group, and error bars indicate SD. Data were analyzed using one‐way ANOVA followed by Dunnett's post hoc test.

Article Snippet: JSH 23 (S7351) and PD98059 (S1177) were purchased from Selleckchem (Houston, TX, USA).

Techniques: Activation Assay

Journal: Physiological Reports

Article Title: TLR4 signaling cascades leading to increases in albumin permeability, nephrin ectodomain shedding, and apoptosis in MPC ‐5 podocytes

doi: 10.14814/phy2.71147

Figure Lengend Snippet: Role of intracellular signaling cascades in TLR4‐mediated apoptosis in podocytes. CRX527 exposure for 72 h causes an increase in apoptosis as indicated by an increase in cleaved caspase‐3. This effect persisted in the presence of the Erk pathway inhibitor PD98059 (a), but apoptosis was reduced or blocked in the presence of the ROS quencher TEMPOL (b), and in the presence of the NFκB pathway inhibitor JSH‐23 (c). In these graphs, points on the bar graphs indicate measurements from different immunoblots, horizontal bars indicate the mean of the group, and error bars indicate SD.

Article Snippet: JSH 23 (S7351) and PD98059 (S1177) were purchased from Selleckchem (Houston, TX, USA).

Techniques: Western Blot

Journal: iScience

Article Title: Downregulation of SPRED1 drives keratinocyte hyperproliferation in psoriasis via activation of the ERK-RSK1 signaling axis

doi: 10.1016/j.isci.2026.116812

Figure Lengend Snippet: SPRED1 regulates NHEK cell proliferation via indirect interaction with ERK (A) Co-immunoprecipitation (CoIP) analysis of SPRED1-ERK interaction in NHEK cells transfected with pLVX-Flag-spred1. (B) qRT-PCR analysis of ERK mRNA expression in five experimental groups treated with ERK agonist Ro67-7476 or inhibitors PD98059/SCH772984. Statistical significance was assessed by one-way analysis of variance (ANOVA). (C and D) Western blot analysis of p -ERK protein expression. Statistical significance was assessed by one-way analysis of variance (ANOVA). (E and F) Immunofluorescence staining of p -ERK. Statistical significance was assessed by Brown-Forsythe corrected ANOVA. Scale bars, 20 μm. (G and H) CCK-8 assays for keratinocyte viability in five groups. Statistical significance was assessed by one-way ANOVA followed by Bonferroni correction. (I and J) EdU staining for keratinocyte proliferation. Statistical significance was assessed by one-way analysis of variance (ANOVA). Scale bars, 200 μm. (K) qRT-PCR analysis of PCNA mRNA expression. Statistical significance was assessed by one-way analysis of variance (ANOVA). (L and M) Western blot analysis of PCNA protein expression. Statistical significance was assessed by one-way analysis of variance (ANOVA). (N and O) Immunofluorescence staining of PCNA. Statistical significance was assessed by Brown-Forsythe corrected ANOVA. Scale bars, 40 μm. All experiments were performed in triplicate. N = 6 for all. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: ERK inhibitor PD98059 , MCE , Cat No. HY-12028.

Techniques: Immunoprecipitation, Transfection, Quantitative RT-PCR, Expressing, Western Blot, Immunofluorescence, Staining, CCK-8 Assay